FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14461584
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC38900.058.17620511
TTCCGAT42150.053.6890110
CCGATCT42500.053.41123212
CTTCCGA44900.050.629629
TCTTCCG47500.047.7835438
CGTGTGC54050.042.8216971
CGATCTA9400.038.17000213
CGATCTT28600.028.22711413
CGATCTG11700.027.12805413
GTGCTCT88450.026.167474
GTGTGCT92500.025.5065332
TGTGCTC96950.023.8376833
GCTCTTC98850.023.7284476
CGACGCT1752.419256E-1023.6526761
CGATCTC50000.017.24990313
TGCTCTT141000.016.7819375
CGACGCC1653.8527873E-516.7241131
CGTTGGA47300.016.0434821
CTCTTCC159150.015.0634427
AAACGGG57000.013.8581967
Produced by FastQC (version 0.11.5)