FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15089356
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT32950.065.248781
GACGCTC38200.056.7240142
TCCGATC40800.054.53896710
ACGCTCT43050.050.8103563
CCGATCT43800.050.72465511
TTCCGAT44300.050.152149
CTTCCGA44900.049.7124638
CGCTCTT48750.044.869454
TCTTCCG49750.044.7274327
CGATCTA9250.032.0748112
CGATCTT31650.026.1604312
CGATCTG12450.024.66198512
GCTCTTC102300.022.1577635
CGTTGGA46500.016.6239741
CGATCTC57300.014.81111812
ACGGGAT32350.014.2901869
CTCTTCC166000.013.77975756
CGTCCGA1800.001404589913.4171946
AACGGGA45550.013.4057858
AAACGGG54600.013.3320587
Produced by FastQC (version 0.11.5)