FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15089356
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC34750.052.21811711
CCGATCT37700.048.4069212
TTCCGAT37600.048.3508810
CTTCCGA40350.045.05419
TCTTCCG44550.041.1146628
CGTGTGC48400.038.912711
CGATCTA7650.035.1756513
CGATCTT25300.024.54488213
CGATCTG11850.022.41719813
GTGCTCT86450.021.7857174
GTGTGCT88200.021.3534582
GCTCTTC93150.020.514986
CGACGCT1851.1816155E-820.5099751
TGTGCTC94700.019.9970783
CGTTGGA47550.017.192621
AAACGGG54100.014.1548637
AACGGGA42400.014.0744098
TGCTCTT136950.014.0041565
CGATCTC53100.013.64374913
GAAACGG55750.013.6120016
Produced by FastQC (version 0.11.5)