FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14422265
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT23750.064.953311
GACGCTC27950.054.6893162
TCCGATC29650.052.12650710
CCGATCT32350.047.88255311
TTCCGAT32800.047.4359939
ACGCTCT33050.046.9765323
CTTCCGA33750.046.2029768
TCTTCCG36150.043.517297
CGCTCTT36300.042.6756024
CGATCTA8450.030.62032912
CGATCTT23900.022.08507212
GCTCTTC84350.018.9380745
CGATCTG11650.017.17541912
CGTTGGA44250.015.052251
CGATCTC46600.013.17769212
ACGGGAT30700.013.147799
AACGGGA41250.013.1304918
AAACGGG50550.012.5574647
GAAACGG51500.012.5277856
CGGGATT30400.012.48315510
Produced by FastQC (version 0.11.5)