FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15618815
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC98400.053.3599111
TTCCGAT99300.052.91068610
CCGATCT100000.052.50631712
CGTGTGC101950.052.1062771
CTTCCGA101050.052.0273449
TCTTCCG105400.050.1722648
CGATCTA23250.047.03796413
CGCCGTA3050.044.11419759
CGATCTG27350.036.95919413
GTGCTCT146350.036.4395754
GTGTGCT149850.035.772632
CGATCTT47600.035.5141413
TGTGCTC154550.034.5508353
GTCGCCG3900.034.4986857
GCTCTTC159900.033.3516736
CGACGCT4650.031.8986321
TCGCCGT4700.030.82860858
TGCTCTT198350.027.0603875
CTCTTCC222500.024.3096247
CGATCTC73650.020.89172713
Produced by FastQC (version 0.11.5)