FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13381941
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT78400.067.178431
GACGCTC84050.062.566412
ACGCTCT87150.060.6881453
TCCGATC88300.059.77718710
TTCCGAT89250.059.102259
CCGATCT90500.058.32404311
CTTCCGA92400.057.2740828
CGCTCTT92750.056.577584
TCTTCCG95250.055.7052467
CGATCTA20050.048.52208712
CGATCTG22750.040.6404212
CGATCTT45100.040.46540512
GCTCTTC144600.036.934465
CTCTTCC202650.026.473596
CGATCTC69500.023.28052512
TCGACGC1502.8938302E-822.99923917
CGACGTC1704.429239E-922.3323881
CTCGACG1959.367795E-1021.23006816
CGTTGGA36650.017.89251
AACGGGA35900.015.3755368
Produced by FastQC (version 0.11.5)