FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13381941
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC86500.056.19373711
CCGATCT87250.055.6328712
CTTCCGA89200.054.72229
TTCCGAT89500.054.3091310
CGTGTGC92050.053.9231031
TCTTCCG93200.052.369898
CGATCTA20850.047.3239113
GTGCTCT128300.038.526314
GTGTGCT129900.038.2110982
CGATCTG20750.037.9085713
TGTGCTC133950.037.0815283
CGATCTT43250.037.01284413
GCTCTTC140600.035.229546
CGCCGTA2000.029.32477659
CGACGCT3450.028.9945771
TGCTCTT174500.028.4448245
CTCTTCC204400.024.45287
CGATCTC68150.023.0338313
CGTTGGA35800.017.2467751
GTCGCCG3551.8189894E-1216.52050657
Produced by FastQC (version 0.11.5)