FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13519540
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC15900.042.0920911
CCGATCT17450.038.35381712
TTCCGAT19200.034.67719310
CTTCCGA21000.031.5393059
TCTTCCG24250.027.453228
CGTGTGC28750.024.8356421
CGATCTA5800.020.22447813
CGTTGGA41800.017.8246231
CGTCCGA1907.041941E-616.339246
ACGGGAT29300.015.8941149
AACGGGA39000.015.5666728
AAACGGG48350.014.4112817
CGATCTT17550.014.15408513
CGACGCT2202.819731E-514.1111581
CGGGATT29650.013.496510
GGAAACG51300.013.38069255
GAAACGG51450.013.2746386
CGATCTG9000.011.50019413
GTGCTCT62400.011.4427024
GTGTGCT64700.011.4091262
Produced by FastQC (version 0.11.5)