FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15591390
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT22300.064.379571
GACGCTC27450.052.2912
TCCGATC28050.050.7954610
CCGATCT30400.046.75535611
ACGCTCT31000.045.9655343
TTCCGAT31450.045.7428449
CTTCCGA33750.042.6255538
CGCTCTT36200.039.6486784
TCTTCCG37150.038.5386927
CGATCTA7750.028.04442612
CGATCTT26150.019.65722812
AGGCGAA13200.018.562821
CGTTGGA45350.018.03561
CGATCTG12050.017.75057412
CGGCGAA2551.364242E-917.5939251
GCTCTTC95000.015.4714125
GGCGACG2353.2194348E-614.6827752
AAACGGG52850.014.2957477
ACGGGAT33200.014.1321599
AACGGGA43550.014.1006648
Produced by FastQC (version 0.11.5)