FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13575088
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT13800.060.2675171
TCCGATC17550.048.5542910
GACGCTC17800.046.910182
TTCCGAT20650.041.59949
CCGATCT20500.041.23063311
ACGCTCT21950.038.6667023
CTTCCGA22900.037.512128
CGCTCTT24450.034.2897224
TCTTCCG25150.033.7446447
CGATCTA5950.021.45322212
AGGCGAA11250.016.5648161
CGTTGGA45750.016.2178291
CGATCTT18900.014.78532812
AAACGGG52400.014.2868417
AACGGGA43400.014.2288918
GAAACGG53050.013.9826556
GGAAACG53800.013.2746975
CGGCGAA2102.991574E-413.1466791
CGATCTG9550.013.00489612
CGACGTC1600.00999031312.9412611
Produced by FastQC (version 0.11.5)