FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13575088
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC13400.041.70678311
CCGATCT15800.035.37183812
TTCCGAT16850.033.3716610
CTTCCGA19100.029.981859
TCGCGTA500.00773727927.60039337
TCTTCCG21750.026.6445338
CGTGTGC27600.022.4966751
CGTTGGA44950.017.803821
ACGGGAT29650.017.8012499
CGATCTA5450.017.72463213
AACGGGA41700.016.2962728
AAACGGG52000.015.5891567
CGGGATT27300.014.78472710
GAAACGG51900.014.68858056
GGAAACG53750.013.9263135
CGATCTT17500.013.60275113
CGGGATA8400.011.90991810
AACGGGT11650.010.6594478
TACGCCC4552.3170105E-710.61561433
GTGCTCT56750.010.5156264
Produced by FastQC (version 0.11.5)