FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16844806
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC24000.044.27247211
CCGATCT26700.040.05437512
TTCCGAT27800.038.09618410
CTTCCGA28400.037.654189
TCTTCCG33150.032.0490468
CGTGTGC37500.029.1586151
CGATCTA8050.020.99992613
CGTTGGA51550.017.7319341
AACGGGA48050.015.7216688
CGATCTG11500.015.29994613
GAAACGG61700.014.9267426
AAACGGG61050.014.8599737
CGATCTT24150.014.71423213
ACGGGAT36300.014.5396739
GGAAACG62300.014.3954155
GTGTGCT84900.013.6918182
GTGCTCT81500.013.6704814
CGGGATT34500.013.59902410
TGTGCTC91000.012.5465843
GCTCTTC92200.012.42076
Produced by FastQC (version 0.11.5)