FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6429176
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC64400.061.39202511
CCGATCT64750.061.0073712
TTCCGAT65450.060.56573510
CTTCCGA65900.060.2568639
CGTGTGC66850.059.7669261
TCTTCCG67050.059.2224548
CGATCTA14500.054.0100413
CGATCTG13200.050.44289413
CGATCTT25550.047.93514613
GTGTGCT83950.047.794992
GTGCTCT83500.047.7629744
TGTGCTC85950.046.562053
GCTCTTC88200.045.373186
TGCTCTT106400.037.806825
CGCCGTA1500.036.80093459
CGATCTC37800.034.6823413
CTCTTCC116800.034.6174137
CGACGCT1052.332672E-829.5737881
TCGCGTT951.051921E-525.4205118
TCGCCGT2350.023.48977558
Produced by FastQC (version 0.11.5)