FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30957599
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA29778800.069.589521
GGACTAG30152750.068.72912
GACTAGC30189250.068.644243
ACTAGCC30115650.068.564174
CTAGCCT30103900.068.519625
TAGCCTT30090750.068.337096
AGCCTTA30016250.068.309917
GCCTTAT30028100.068.246598
CCTTATT30057750.068.1887369
CTTATTT30167150.067.93259410
TTATTTT29945350.067.8541911
TATTTTA30030550.067.712212
ATTTTAA30011500.067.6983813
TTTTAAC30050400.067.495914
TAAAACG3955900.066.98060637
CTAAAAC30141350.066.9534936
TAAAACT8746650.066.9517137
AAAACGC1664350.066.9477638
TCTAAAA30162550.066.9461735
AAAACTC2996900.066.9434738
Produced by FastQC (version 0.11.5)