FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23492017
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA22479250.069.587551
GGACTAG22751800.068.738442
GACTAGC22781600.068.644793
ACTAGCC22738050.068.557044
CTAGCCT22716350.068.519395
TAGCCTT22712650.068.334996
AGCCTTA22661400.068.310267
GCCTTAT22671700.068.2459268
CCTTATT22689200.068.198659
CTTATTT22756800.067.98283410
TTATTTT22613300.067.93085511
TATTTTA22691200.067.766512
ATTTTAA22677500.067.7482213
TTTTAAC22693200.067.56370514
AAAACCG2396150.067.08395438
CTAAAAC22736300.067.0622636
TCTAAAA22761050.067.0566935
CTCTAAA22866250.067.0529534
AAAACGC1249700.067.04722638
TAAAACT6594700.067.04053537
Produced by FastQC (version 0.11.5)