FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26896392
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTA25895550.069.581671
GGACTAG26208500.068.7419742
GACTAGC26239150.068.660893
ACTAGCC26185950.068.578184
CTAGCCT26170600.068.549165
TAGCCTT26169700.068.382456
AGCCTTA26122250.068.346767
GCCTTAT26134800.068.279678
CCTTATT26158600.068.228649
CTTATTT26234900.068.0056210
TTATTTT26090500.067.9301711
TATTTTA26144800.067.8227512
ATTTTAA26131800.067.8024613
TTTTAAC26151500.067.6357414
CGAAAAC13950.067.3983836
AAAACCG2759700.067.20473538
TAAAACT7572400.067.20289637
CTAAAAC26194800.067.20193536
TCTAAAA26215600.067.19626635
TAAAACC13503850.067.194437
Produced by FastQC (version 0.11.5)