FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13890152
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN139800.10064684677316706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC99350.021.6947848
TATACTG138200.019.622545
TACACTG119850.019.2747755
TCTATAC77550.017.3549163
TGCACTG197850.017.0231595
ATACTGT167200.017.0200546
CTATACT79450.016.602774
CTTACAC78950.016.1142353
TAGACAG153050.015.6623875
GTATAGG54850.015.0167471
GCACTGA164250.015.0020536
CTGATCA136550.014.9999859
GTGTAGA90650.014.9960061
CTACACT87600.014.9052324
CTAGGAC67500.014.6813563
TCTAGAC68200.014.1379473
TACACAG163600.014.1203045
GTCCTAG67450.013.9985981
GTATTAG73500.013.9396361
GTGTAGG86600.013.9188071
Produced by FastQC (version 0.11.5)