FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13890152
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN204450.14719061389680976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC96800.021.0164538
TATACTG125450.020.2163665
TACACTG119800.018.6562445
TGCACTG190450.015.9166545
ATACTGT161150.015.7797586
CTACACT88500.015.50024
CTGATCA131400.015.1260449
GTCCTAG67000.014.9957351
CTATACT75500.014.8011774
CTAGGAC65150.014.585073
GTATTAG76900.014.545931
CCTATAC47450.014.2435993
GTATAGG56300.014.1576081
GTATATA244700.014.0421871
TATACAG113500.014.0318735
TCTATAC77550.013.8924443
TAGACAG149000.013.6528045
CACTGAT152200.013.6300447
CTAGCAC70250.013.6214793
GTGTAGG90900.013.5583011
Produced by FastQC (version 0.11.5)