FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9770513
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN138140.1413845926002043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG74700.017.8287725
GCTATAC30000.017.7571833
CTAGACT33750.017.4356354
GTGCTAG42000.016.9766861
CTATACT46800.016.8091554
GTCCTAG36750.016.702531
CCTACAC56350.016.049293
CCTATAC28600.016.027383
CCTAGAC40650.016.0002083
GCTAGAC31250.015.8575783
GTCCTAA35650.015.8265491
ACTGATC55850.014.7520388
GCTACAC49250.014.3382273
GTGCTAC42050.014.3024391
TCTATAC48000.014.1954223
GTGCTAT47650.014.0528761
TGCTAGA49600.013.9916522
CCCCTAG40200.013.8810021
GTCCTAC34800.013.7187921
CTATACC33450.013.518074
Produced by FastQC (version 0.11.5)