FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7934114
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG117320.1478678022524002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC11955.674756E-79.0141873
TTCCGAT14708.5226915E-56.8438682
GGATGCG35705.4974844E-65.835162145
GCCTAAT24959.3054696E-55.18839361
TTCCGCC31700.00124524115.187995145
CCGATCT18350.00891601855.0855824
Produced by FastQC (version 0.11.5)