FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6353595
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAC14900.0048903545.80659771
GTTTAAT47900.05.56920151
GTTGTAC27151.1057107E-55.31112961
GTTTAAG32653.449095E-75.29974271
CTTAAGT29952.0131993E-65.2960561
GTTAGGT19200.0025110245.25718831
GTTAGAT20750.00117133485.2119461
CGTGCGC27250.00737445855.168888145
GTTTAGG29801.0272048E-55.0807731
Produced by FastQC (version 0.11.5)