FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12083472
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG737840.6106191995148414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT16701.0458025E-67.34444571
CGCGATC15301.3778974E-46.5968372
GTTTCAC109700.06.5111121
TGCACTC159300.06.42645842
AGGGGGG264250.06.2797081
GCACTCC148850.06.19878153
CTTTCGT25305.0046765E-75.98860071
GCTTCAC121800.05.8642781
GTTCTAT62200.05.6837151
GTTCGCT17900.0010281425.642891
CTGCACT213350.05.61360741
CTTTAAC82300.05.6105661
GCGATCT31201.3485806E-75.54500343
GTTCGTT15850.009606575.4623381
GTTTTAC86450.05.42469071
CACTCCA204250.05.29401544
GCTAAAC44355.2204996E-105.20574571
ACTCCAG245850.05.1898795
GTTTCAT117550.05.1556481
GTTCAAC63650.05.1008281
Produced by FastQC (version 0.11.5)