FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8016240
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG7800.00371196338.321917
TTCGACT18650.00564483466.2867613145
TTGAATC45950.06.1229762
GTTAATT45400.05.8841231
GTTTAAC33202.2901077E-95.87166831
GTCAAAA67700.05.7589191
GTTTTTT140950.05.73705151
GTTGAAT52500.05.63845871
GTAAATT57250.05.54897831
GTAAAAT79350.05.459341
GTTTTAA92750.05.449041
GTCTAGT19150.00238818375.2783131
GTTTAAT54251.8189894E-125.19039871
CTCAAAT65450.05.1847051
AATTTAC56801.8189894E-125.08043055
TTTAGAC38406.877053E-85.0724292
GTTTAAG38808.556344E-85.0242111
Produced by FastQC (version 0.11.5)