FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10629296
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAAT72100.06.5111991
GCTAGAT25357.712333E-86.26797961
GTTTTAA117000.05.80262951
GGTTAAC25754.241134E-65.60964781
GTTTTTT181950.05.55723571
GTTAAAA99950.05.49178651
GTTTTAT117700.05.39994141
GTTAGGC25452.0738811E-55.39198451
GTAAAAT103750.05.36025521
GGAAAAT158350.05.3364281
GTTAGGT28504.427391E-65.32178451
GTTTAAT74050.05.2668571
GCGAAAT19200.00245737545.26634931
GTGAAAT105800.05.1881291
GCAATCC50853.092282E-115.10915333
GTTAAAC42655.3751137E-95.080251
Produced by FastQC (version 0.11.5)