FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8931793
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG373690.41838184113760807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG161850.07.44085261
CCGTTGC26950.00568486335.285164145
GCGAAAA19950.00404142085.0606241
Produced by FastQC (version 0.11.5)