FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4053510
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113970.2811637321728576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGCT10651.03918486E-48.1433061
TCGGCTC17751.3899396E-46.10182432
GTTAGAT14300.00301105976.064771
GTCTAAG13200.0088071286.02265361
TAGCATA14550.0037317915.94931559
GTTTAAT31805.155016E-95.90901851
GTCCAAT15450.0071115765.6133471
AGGGGGG172450.05.49006181
TTAATCT28003.258785E-65.411363
GTTTGAA39704.811227E-95.2793031
GTTCAAT19400.00277341865.21549371
GGGACTA19700.0034385625.1265034
Produced by FastQC (version 0.11.5)