FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12320476
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAATC94450.06.49331862
GTGAAAT127150.05.3956661
GGAAAAT197850.05.29260831
GTAAAAT128350.05.28895331
GTTAAAT87450.05.2851581
CTTTCGT31304.506701E-65.0759361
GGGGGGG1029800.05.049684145
GTTAACT54609.094947E-125.02607251
Produced by FastQC (version 0.11.5)