FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8909593
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161970.18179281590079366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATA10256.2519027E-47.74396471
GTTTAGT41501.8189894E-125.9118761
GTTAGAT34553.4868208E-85.43024971
GTTGAAT73550.05.39603231
GCCTAGT26403.8687744E-55.19330931
GTGAAAT107400.05.17345861
GTCTAAG31104.051659E-65.10454231
GGTCAAT48101.6916601E-105.1006831
GTTTTTT203350.05.00344851
GTTTTAG69250.05.00168131
Produced by FastQC (version 0.11.5)