FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7809332
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218760.28012639237261266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG308650.05.65485531
AACGCTC15650.008935955.498365
GTTAGAT23904.812157E-55.40948721
GGTCGAT18250.0083105235.11637071
GTCAGAT44958.458301E-105.1133061
GCCTAAT21150.00158783145.09404041
Produced by FastQC (version 0.11.5)