FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7680484
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105280.1370746947718399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAT9650.00300042567.4680891
GTCGGAT11300.00178786027.0153771
AAATCCG16654.197935E-46.05263044
GCTAGAT22051.2572316E-55.88302561
ATCGTGA19003.6652872E-45.6828746
GCGAAAT15650.00845785.5259091
CTTAAAT69400.05.29599431
GTCAAAT52952.0008883E-115.03584771
GTTGAAT58950.05.01229761
Produced by FastQC (version 0.11.5)