FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5525859
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAT17503.2463504E-87.84561631
TCGAATT11850.00648857147.8207245145
GTTTAAT38004.5474735E-115.8950781
GCTAAAC19003.4773248E-45.70491461
GTTCAAA43252.5283953E-105.34657141
GTCAAAA43352.6921043E-105.33423761
GGATTAC41202.215529E-95.2602435
GTGAAAT56450.05.24845361
GTTTAGA26203.3301316E-55.2403921
CTTAAAT44052.2628228E-95.0854251
Produced by FastQC (version 0.11.5)