FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6067735
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64390.10611867525526411No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG10500.00169616049.07257145
GCACGAC18256.355736E-46.9597793145
ATCGAGG23852.9948266E-46.21322145
CGCGAGG30900.00141537595.1381865145
Produced by FastQC (version 0.11.5)