FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5825048
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC9251.2732926E-811.6855931
CCGATCT14156.0598304E-67.63899142
GTTCGAA8500.0080944787.6300051
AAACGCT17809.719212E-45.667786
GCTTAAC15600.00817144855.5431661
GTTCAGT40508.465577E-95.15993451
GTCTAAG18600.0099786395.03653241
Produced by FastQC (version 0.11.5)