FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9671136
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC17800.030.8480191
CCGATCT25400.023.3234942
CGATCTA10859.331416E-1011.3196413
CGATCTG30751.8189894E-127.04838473
CGATCTC39853.6379788E-125.98272563
CGATCCC25700.00495750045.3471613145
Produced by FastQC (version 0.11.5)