FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8796494
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG438980.4990397310564868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG160450.010.4174841
GCGAAAA20505.4672273E-76.6775061
GTTCGAT12150.00384404466.52275371
GCGGATT16300.00211594885.74606231
GCGGGTT25854.7411886E-65.57421881
GGGGGTT117950.05.00357773
Produced by FastQC (version 0.11.5)