FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6158594
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGT23602.7488422E-67.437514145
GGATCGG19350.00613653856.2363644145
AGCTCGG53201.3224053E-95.773859145
GTTTAGT24808.58789E-55.2148381
AAGCGCC29650.00302119955.1799207145
GGCAATT32251.6125268E-65.12410641
GTTAAAT53555.456968E-125.098521
Produced by FastQC (version 0.11.5)