FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8323551
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291530.35024714812223773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG136500.07.08073041
GCGAAAT15500.00114327346.0494741
GCGAAAA18101.8432355E-45.97748661
TGCTCGC32455.394501E-55.6967278145
GGGGGTT104050.05.4715073
GCTTAAC22851.3316941E-45.3662231
Produced by FastQC (version 0.11.5)