FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3915736
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC18900.00512191056.3454485145
GTTTAAT23851.7414277E-76.3347391
CAATCCA24603.166715E-76.1364294
AAATCCA47750.05.72060254
GTTGATT20701.9527656E-45.56093031
AATCTTA21252.873606E-45.4124335
GTTTTTT61200.05.4075961
TTTACAC19957.2345836E-45.40307767
AAAATCC39003.5415724E-95.3450073
AATTTAC23452.0647656E-45.2111995
TGCAATC20700.00117999185.20891052
GTCAGAA35959.028736E-85.2032211
GCAATCC19500.00316474285.16069653
GGAAAAT51851.0913936E-115.13393971
GTCAAGT21100.0015046855.1145411
CTAAAAT32401.7198236E-65.1071741
CTTAGGA19800.00378648035.08698751
CTTAAAA42455.2514224E-95.08441971
AAAAAAC58650.05.0250672
AAAATTT70100.05.0245013
Produced by FastQC (version 0.11.5)