FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3915736
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73440.18755094827639043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGG12801.37005245E-57.85813866
TCGAGCG8300.006717627.7905127
GCTAATT15001.094037E-46.71418571
GCTAAAT15151.2457679E-46.6477081
TTAAGCC12800.0068084136.1742527
GTTTAGG14750.00447900735.85255861
ATAAGTG17950.00112282525.6035758
GCTATTT22501.0978016E-45.4352931
GCTTATT19000.00226376585.3006731
GTGAAAT38301.2572855E-85.25915341
GGCAACC29400.00255811215.2490506145
GGTGAAT28906.128779E-65.2273081
GTTAATT19800.00379095765.08650451
Produced by FastQC (version 0.11.5)