FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7639105
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAAT14850.0044522137.172025145
GCGAGAC27401.20599E-96.58987051
GTTTAAA69550.05.91922861
GTTAAAT52500.05.915571
GTCTAAC16600.00255229775.65617281
GTTAGGT23152.6515498E-55.6157651
GTTTAGT27502.2999175E-65.5153621
GTTGAAC40652.764864E-105.50793171
GTTTAAT51700.05.4483061
CTTAAAT57200.05.42951
GTTCAAA60300.05.38992361
GTTTTTT136600.05.3402071
GTTTAAG38101.0100848E-85.30787231
GTAAAAT82950.05.13715931
GTCCAAT33756.587943E-75.13599821
GTAATTT72350.05.0911871
TTAGGTA34158.568022E-75.0706248
CTAAGAT33202.5768986E-65.00353771
GCTAAGT26001.6606023E-45.00019071
Produced by FastQC (version 0.11.5)