FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5684026
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69960.12308177337682832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGC6850.004587745311.45819145
ATATCGG10000.00724409668.970126145
GCATGCG20851.9703792E-46.991105145
GCGTCGG27057.192031E-45.8032236145
AGATAGG29854.1122225E-45.6345015145
GTCCCTA20551.837937E-45.5846691
AGAGCGG46752.157647E-65.276545145
TGCGCGG32850.00148366585.1199346145
GGCAAAT45009.0403773E-105.1006641
GTTAAAT48552.6011548E-105.02318241
Produced by FastQC (version 0.11.5)