FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5915683
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120950.20445652682877022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTT19501.7021539E-66.6514521
AGATAGG28609.407711E-55.8507495145
GTTAAGT21304.669508E-55.75105951
GGGATCG22450.0074442745.590137145
GTTCATA24456.555456E-55.30483871
AGAGCGG63051.6916601E-95.142025145
Produced by FastQC (version 0.11.5)