FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7569939
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95140.125681329796713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGT12750.0061609226.2341511
GTCTAAT22701.1735261E-45.4115031
CTTAGTT30052.0564348E-65.2902111
GTTAATT48704.3655746E-115.19318631
GTTTAAA78050.05.0919551
GTTAAAT59950.05.06238171
GTTTACT41401.3582394E-85.0616541
GCTAAAC24353.3496597E-45.044811
Produced by FastQC (version 0.11.5)