FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4797272
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAAC12250.00413200266.47769451
GTTAGAT14050.00252360766.1612491
CCTAAAT24752.1479555E-65.829331
ACTCTAG21304.589763E-55.75748976
GTTTAAC18100.00118539745.5797321
TTAGTCT18500.00156288665.45908933
CTAGATT20007.10662E-45.4103471
GCAATCT24607.0925686E-55.27838753
GTTTATT47059.094947E-115.2129451
GTTACAT21250.0015953995.0920911
CTAAGAT19850.00377943585.0878161
ATTTGCC37301.9250729E-75.02838336
GTCAATT20150.00455421775.0120671
Produced by FastQC (version 0.11.5)