FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6134665
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA17651.3563942E-46.1128681
ATCGGAA19307.155388E-55.9626582
ACCGGTG22550.0013706565.9585304145
AGTCTAG20201.380633E-45.69718075
GTCCAAT25654.2553092E-65.60842751
GGGGGGG540250.05.5672483145
GTTAGGT18250.0013656265.51777031
GTTTTTT87550.05.3401771
CCCGACG25350.00549507935.3003893145
AGAACCG25600.0061652945.2486277145
CGGGGGG128700.05.1397715145
GCCAATT28753.1074487E-55.00369261
Produced by FastQC (version 0.11.5)