FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6134665
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGCG14600.0043170327.1947036145
CCGTCGG24600.00318724175.5510354145
TTGGGCG22850.0086160235.516464145
AATTGAG38152.0227162E-95.4672625
GCGAGCG33651.5731886E-45.3067627145
GGGTCGG46851.4210418E-65.156844145
GTTAAAT42354.783942E-95.1034951
GTCAACC29150.00419868345.0449314145
Produced by FastQC (version 0.11.5)