FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7912155
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135290.1709900779244087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG71400.020.655155
ACTGATC49100.017.8097528
CTATACT42350.016.3327584
CCTATAC25650.016.2822133
ATACTGT88950.016.1397786
GTATAGG28500.014.8959741
TGCACTG96700.014.8461655
GTATTAG41300.014.7073141
TCTAGAC35150.014.2950693
TACACTG66950.013.8406315
CCTACAC43250.013.7301773
TAGACAG75900.013.6701565
GTCCTAG33950.013.6590031
GTATATA133750.013.5753631
TAGACTG44050.013.3325955
TAATACT65600.013.3293214
TAGTACT35050.013.2183554
GTACTAG22300.013.1797781
CTAGGAC35200.013.1624443
GTATAAG41350.012.9520581
Produced by FastQC (version 0.11.5)