FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6739662
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269560.39996071019585255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA35800.07.64875464
GGGGGTT90550.06.92326973
GACTAGT13150.0087321466.02777536
CGGGTTT17156.047008E-45.8827432
GCGAAAA16100.00180350715.8235121
AGGGGGG108150.05.73507931
GGGTTAA34154.654794E-95.6972225
GTTCAAC32753.649966E-75.28527361
GGGGTTT81800.05.109345
GTTAGTT24153.0483076E-45.07690761
CCGAAAA24203.14343E-45.06641861
GTTTGAA70300.05.02699231
Produced by FastQC (version 0.11.5)