FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822469
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8005.456968E-1215.3382531
ATCGGAA9853.1415766E-810.9918812
TCCGATC7700.00327971868.43662551
GTTAAAC22853.4087134E-66.00183961
GTAAAAT54600.05.94890261
ACTTACG14700.0041551985.89224626
GTTAGGT14800.00448066935.8524341
AAATCTA42303.6379788E-125.80170354
TCGGAAG24058.109035E-65.70237163
AATCTAG20351.4595927E-45.67508755
GCCAAAT37306.157279E-95.4183391
GTTCAAA37808.476491E-95.3466681
GTTTAAT38651.4426405E-85.22908261
GGATTTA29056.336735E-65.21783264
GTAAAGT27701.5365293E-55.21155361
AATCTAC21100.00144108735.1312815
GTTGACT28202.0952797E-55.119151
GTCCAAT19800.00363608875.10363771
ATTCAAC32751.9935833E-65.06913046
GTCCAGT46001.376975E-95.0212191
Produced by FastQC (version 0.11.5)