FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005073469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005073469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5716497
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGT7550.00281542738.5802388
CGAAAAT15853.0122095E-56.81543831
GTTTAAT55150.06.13739541
GTCGCCT20152.014921E-56.0758311
GGCAATT37752.5283953E-105.72316271
GTTAAAT55000.05.4994391
GTTTTGT90700.05.478651
GTCAGTT39508.1490725E-105.46960451
GTCTAGA21302.8955235E-45.40968751
GTCAAAT43301.5788828E-95.1559131
GTCTAAT21050.00143928585.13181451
CAGACGG28600.0091694715.0731587145
GGCTAAT25701.4487961E-45.0439551
GTCAATT35754.2807005E-75.0361161
GTTTTTT137500.05.02805851
GTTGAAT48752.6193447E-105.02268651
Produced by FastQC (version 0.11.5)